Main Page
Primer Designer
Upload gene sequence (mRNA or CDS) (.fasta):
Upload
Primer Length: Min - Preferred - Max (nt):
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Primer Annealing Temperature: Min - Preferred - Max (nt):
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Primer GC Content: Min - Max (%):
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Minimum coverage of dsRNA region (%):
Add overhang to 5' end of Forward primer:
None
T7 Promoter
SP6 Promoter
T3 Promoter
Add overhang to 5' end of Reverse primer:
None
T7 Promoter
SP6 Promoter
T3 Promoter
Generate additional primers without 5' overhangs
Design Primers