Main Page
dsRNA designer
Paste target gene sequence:
Or upload .fasta file containing target genes:
Upload
(Optional) Upload transcriptome for off-target prediction in addition to selectable species in the Safety/Off-target section (.fasta):
Upload
Click here to automatically fetch non-target transcriptome from NCBI
Efficiency
Correct sequence direction based on longest ORF
Restrict target site to ORF
siRNA length (nt):
19
20: some lepidopterans
21: recommended for most pests e.g., coleopterans
22
23
dsRNA length range:
-
bp
Buffer zone flanking selected dsRNA region (bp):
bp
Safety & Off-target
Select category and then click on species for off-target prediction:
Recommended Package
Pollinators
Natural Enemies
Microorganisms
Mammalians
Plants
Model Organisms & Others
Species selected for off-target prediction:
Essential gene off-target priority
Mismatch tolerance per siRNA for off-target prediction:
Do not allow mismatches
up to 1 mismatch
up to 2 mismatches
Adjust the slider to prioritize efficiency vs safety/off-target
Prioritize efficiency prediction
50%
Prioritize safety/off-target prediction
50%
Design dsRNA